Deleted
Sample Discontinued
Experience: None
No Sequence
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Regulatory

Part:BBa_K328002:Design

Designed by: Cristian Delgado   Group: iGEM10_Mexico-UNAM-CINVESTAV   (2010-10-26)

cspA UTR Region


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This is the complete promoter cspA promoter + Cold Box + UTR + SD + DB extracted from E coli dh5a for test about the cold shock response and the ability to control a genetic circuit using it as a cold sensor, is intended to produce proteins after a cold shift and allows us to sintetize thermo-labile proteins also.

Source

PCR E coli dh5a

References

Fang L., Jiang W., Bae W., Inouye M., 1997, Promoter-independent cold-shock induction of cspA and its derepression at 37 degrees C by mRNA stabilization., Mol Microbiol. 23(2):355-64 Hankins JS., Zappavigna C., Prud'homme-Genereux A., Mackie GA., 2007, Role of RNA structure and susceptibility to RNase E in regulation of a cold shock mRNA, cspA mRNA., J Bacteriol. 189(12):4353-8 Xia B., Ke H., Jiang W., Inouye M., 2002, The Cold Box stem-loop proximal to the 5'-end of the Escherichia coli cspA gene stabilizes its mRNA at low temperature., J Biol Chem. 277(8):6005-11